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At baseline, R607H KI/KI and L919X KI/KI mice exhibit alkaline urine and decreased urinary ammonium secretion. (A-F) Urinary pH (A), ammonium/creatine ratio (B), <t>osmolality</t> (C), sodium/creatinine ratio (D), chloride/creatinine ratio (E) and potassium/creatinine ratio (F) comparison between WT and L919X KI/KI mice. (G-L) Urinary pH (G), ammonium/creatine ratio (H), osmolality (I), sodium/creatinine ratio (J), chloride/creatinine ratio (K) and potassium/creatinine ratio (L) comparison between WT and R607H KI/KI mice. Error bars correspond to means±s.e.m. ** P <0.01, **** P <0.0001 using unpaired two-tailed Student's t -test or Mann–Whitney test. Note that the large sample size is due to the analysis of baseline urine samples from all experiments, resulting in 34-37 samples for WT and 35-39 samples for KI mice.
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At baseline, R607H KI/KI and L919X KI/KI mice exhibit alkaline urine and decreased urinary ammonium secretion. (A-F) Urinary pH (A), ammonium/creatine ratio (B), <t>osmolality</t> (C), sodium/creatinine ratio (D), chloride/creatinine ratio (E) and potassium/creatinine ratio (F) comparison between WT and L919X KI/KI mice. (G-L) Urinary pH (G), ammonium/creatine ratio (H), osmolality (I), sodium/creatinine ratio (J), chloride/creatinine ratio (K) and potassium/creatinine ratio (L) comparison between WT and R607H KI/KI mice. Error bars correspond to means±s.e.m. ** P <0.01, **** P <0.0001 using unpaired two-tailed Student's t -test or Mann–Whitney test. Note that the large sample size is due to the analysis of baseline urine samples from all experiments, resulting in 34-37 samples for WT and 35-39 samples for KI mice.
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At baseline, R607H KI/KI and L919X KI/KI mice exhibit alkaline urine and decreased urinary ammonium secretion. (A-F) Urinary pH (A), ammonium/creatine ratio (B), <t>osmolality</t> (C), sodium/creatinine ratio (D), chloride/creatinine ratio (E) and potassium/creatinine ratio (F) comparison between WT and L919X KI/KI mice. (G-L) Urinary pH (G), ammonium/creatine ratio (H), osmolality (I), sodium/creatinine ratio (J), chloride/creatinine ratio (K) and potassium/creatinine ratio (L) comparison between WT and R607H KI/KI mice. Error bars correspond to means±s.e.m. ** P <0.01, **** P <0.0001 using unpaired two-tailed Student's t -test or Mann–Whitney test. Note that the large sample size is due to the analysis of baseline urine samples from all experiments, resulting in 34-37 samples for WT and 35-39 samples for KI mice.
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At baseline, R607H KI/KI and L919X KI/KI mice exhibit alkaline urine and decreased urinary ammonium secretion. (A-F) Urinary pH (A), ammonium/creatine ratio (B), <t>osmolality</t> (C), sodium/creatinine ratio (D), chloride/creatinine ratio (E) and potassium/creatinine ratio (F) comparison between WT and L919X KI/KI mice. (G-L) Urinary pH (G), ammonium/creatine ratio (H), osmolality (I), sodium/creatinine ratio (J), chloride/creatinine ratio (K) and potassium/creatinine ratio (L) comparison between WT and R607H KI/KI mice. Error bars correspond to means±s.e.m. ** P <0.01, **** P <0.0001 using unpaired two-tailed Student's t -test or Mann–Whitney test. Note that the large sample size is due to the analysis of baseline urine samples from all experiments, resulting in 34-37 samples for WT and 35-39 samples for KI mice.
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At baseline, R607H KI/KI and L919X KI/KI mice exhibit alkaline urine and decreased urinary ammonium secretion. (A-F) Urinary pH (A), ammonium/creatine ratio (B), <t>osmolality</t> (C), sodium/creatinine ratio (D), chloride/creatinine ratio (E) and potassium/creatinine ratio (F) comparison between WT and L919X KI/KI mice. (G-L) Urinary pH (G), ammonium/creatine ratio (H), osmolality (I), sodium/creatinine ratio (J), chloride/creatinine ratio (K) and potassium/creatinine ratio (L) comparison between WT and R607H KI/KI mice. Error bars correspond to means±s.e.m. ** P <0.01, **** P <0.0001 using unpaired two-tailed Student's t -test or Mann–Whitney test. Note that the large sample size is due to the analysis of baseline urine samples from all experiments, resulting in 34-37 samples for WT and 35-39 samples for KI mice.
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At baseline, R607H KI/KI and L919X KI/KI mice exhibit alkaline urine and decreased urinary ammonium secretion. (A-F) Urinary pH (A), ammonium/creatine ratio (B), <t>osmolality</t> (C), sodium/creatinine ratio (D), chloride/creatinine ratio (E) and potassium/creatinine ratio (F) comparison between WT and L919X KI/KI mice. (G-L) Urinary pH (G), ammonium/creatine ratio (H), osmolality (I), sodium/creatinine ratio (J), chloride/creatinine ratio (K) and potassium/creatinine ratio (L) comparison between WT and R607H KI/KI mice. Error bars correspond to means±s.e.m. ** P <0.01, **** P <0.0001 using unpaired two-tailed Student's t -test or Mann–Whitney test. Note that the large sample size is due to the analysis of baseline urine samples from all experiments, resulting in 34-37 samples for WT and 35-39 samples for KI mice.
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At baseline, R607H KI/KI and L919X KI/KI mice exhibit alkaline urine and decreased urinary ammonium secretion. (A-F) Urinary pH (A), ammonium/creatine ratio (B), osmolality (C), sodium/creatinine ratio (D), chloride/creatinine ratio (E) and potassium/creatinine ratio (F) comparison between WT and L919X KI/KI mice. (G-L) Urinary pH (G), ammonium/creatine ratio (H), osmolality (I), sodium/creatinine ratio (J), chloride/creatinine ratio (K) and potassium/creatinine ratio (L) comparison between WT and R607H KI/KI mice. Error bars correspond to means±s.e.m. ** P <0.01, **** P <0.0001 using unpaired two-tailed Student's t -test or Mann–Whitney test. Note that the large sample size is due to the analysis of baseline urine samples from all experiments, resulting in 34-37 samples for WT and 35-39 samples for KI mice.

Journal: Disease Models & Mechanisms

Article Title: Urinary sodium wasting and disrupted collecting duct function in mice with distal renal tubular acidosis mutations

doi: 10.1242/dmm.052138

Figure Lengend Snippet: At baseline, R607H KI/KI and L919X KI/KI mice exhibit alkaline urine and decreased urinary ammonium secretion. (A-F) Urinary pH (A), ammonium/creatine ratio (B), osmolality (C), sodium/creatinine ratio (D), chloride/creatinine ratio (E) and potassium/creatinine ratio (F) comparison between WT and L919X KI/KI mice. (G-L) Urinary pH (G), ammonium/creatine ratio (H), osmolality (I), sodium/creatinine ratio (J), chloride/creatinine ratio (K) and potassium/creatinine ratio (L) comparison between WT and R607H KI/KI mice. Error bars correspond to means±s.e.m. ** P <0.01, **** P <0.0001 using unpaired two-tailed Student's t -test or Mann–Whitney test. Note that the large sample size is due to the analysis of baseline urine samples from all experiments, resulting in 34-37 samples for WT and 35-39 samples for KI mice.

Article Snippet: Urine osmolality was measured with the Advanced Instruments Osmo1 Single-Sample Micro-Osmometer (Thermo Fisher Scientific), diluting samples at 1:10 or 1:100 with filtered ddH 2 O. Urine electrolytes were measured by ion chromatography (Dionex Aquion Ion Chromatography System, Thermo Fisher Scientific) with an autosampler.

Techniques: Comparison, Two Tailed Test, MANN-WHITNEY

After a salt-depleted acid diet, R607H KI/KI mice and L919X KI/KI mice are hypernatremic and hyperchloremic. (A,B) Plasma sodium (A) and chloride (B) concentrations from L919X KI/KI mice or WT littermates. (C) Renin gene expression in L919X KI/KI mice or WT littermates. (D,E) Urine osmolality (D) and water consumed (E) by L919X KI/KI mice or WT littermates over the 14-day time course of the diet. BW, body weight. (F,G) Plasma sodium (F) and chloride (G) concentrations from R607H KI/KI mice or WT littermates. (H) Renin gene expression in R607H KI/KI mice or WT littermates. (I,J) Urine osmolality (I) and water consumed (J) by R607H KI/KI mice or WT littermates over the 14-day time course of the diet. Error bars correspond to means±s.e.m. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 using unpaired two-tailed Student's t -test or two-way ANOVA with Tukey's multiple comparison test.

Journal: Disease Models & Mechanisms

Article Title: Urinary sodium wasting and disrupted collecting duct function in mice with distal renal tubular acidosis mutations

doi: 10.1242/dmm.052138

Figure Lengend Snippet: After a salt-depleted acid diet, R607H KI/KI mice and L919X KI/KI mice are hypernatremic and hyperchloremic. (A,B) Plasma sodium (A) and chloride (B) concentrations from L919X KI/KI mice or WT littermates. (C) Renin gene expression in L919X KI/KI mice or WT littermates. (D,E) Urine osmolality (D) and water consumed (E) by L919X KI/KI mice or WT littermates over the 14-day time course of the diet. BW, body weight. (F,G) Plasma sodium (F) and chloride (G) concentrations from R607H KI/KI mice or WT littermates. (H) Renin gene expression in R607H KI/KI mice or WT littermates. (I,J) Urine osmolality (I) and water consumed (J) by R607H KI/KI mice or WT littermates over the 14-day time course of the diet. Error bars correspond to means±s.e.m. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 using unpaired two-tailed Student's t -test or two-way ANOVA with Tukey's multiple comparison test.

Article Snippet: Urine osmolality was measured with the Advanced Instruments Osmo1 Single-Sample Micro-Osmometer (Thermo Fisher Scientific), diluting samples at 1:10 or 1:100 with filtered ddH 2 O. Urine electrolytes were measured by ion chromatography (Dionex Aquion Ion Chromatography System, Thermo Fisher Scientific) with an autosampler.

Techniques: Clinical Proteomics, Gene Expression, Two Tailed Test, Comparison